Selective assay for herpes simplex viruses expressing thymidine kinase
نویسندگان
چکیده
منابع مشابه
Analysis of thymidine kinase mutations conferring acyclovir resistance in herpes simplex type 1 recombinant viruses Running title: Thymidine kinase mutations
49 Text: 1331 Corresponding author: Guy Boivin CHUQ-CHUL, room RC-709 2705, blvd Laurier, Sainte-Foy, Québec, Canada G1V 4G2 Tel: (418) 654-2705 Fax: (418) 654-2715 E-mail: [email protected] Alternate corresponding author : Yan Sergerie CHUQ-CHUL, room RC-709 2705, blvd Laurier, Sainte-Foy, Québec, Canada G1V 4G2 Tel: (418) 654-2705 Fax: (418) 654-2715 E-mail: [email protected]...
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When employed as a transgene reporter, the herpes simplex type 1 virus (HSV1) thymidine kinase gene (tk) is ectopically expressed in mouse testis. The principal testicular mRNA lacks the 5'-end of the tk reading frame. As a result the principal translation products, P2 and P3, are N-terminally truncated. These co-migrate in SDS-PAGE with polypeptides synthesised during HSV1 infection that were ...
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Purified herpes simplex virus thymidine kinase has been used to immunize mice for the production of monoclonal antibodies to the enzyme. Monoclonal antibodies were successfully produced against both herpes simplex virus type 1 and type 2 enzymes. These antibodies should prove useful for detecting the enzyme under a variety of experimental conditions. We also demonstrate that the antibodies can ...
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Herpes simplex virus (HSV) infection of mouse liver after partial hepatectomy was studied. Partial hepatectomy resulted in the rapid onset of cellular DNA synthesis and the appearance of many mitotic figures (peak, 3 days after surgery). Similar changes were not seen in control animals. After partial hepatectomy, the mice were infected with thymidine kinase-positive (TK+) and -negative (TK-) HS...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1979
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.31.2.281-287.1979